SerpinB2/PAI-2 Polyclonal antibody, PBS Only

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• SerpinB2/PAI-2 Polyclonal antibody, PBS Only (16035-1-PBS)

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  WB analysis using 16035-1-AP (same clone as 16035-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16035-1-AP (SERPINB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16035-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16035-1-AP (same clone as 16035-1-PBS)

  IP result of anti-SERPINB2 (IP:16035-1-AP, 4ug; Detection:16035-1-AP 1:300) with Jurkat cells lysate 3600ug. This data was developed using the same antibody clone with 16035-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16035-1-AP (same clone as 16035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16035-1-AP (same clone as 16035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16035-1-PBS in a different storage buffer formulation.

SerpinB2/PAI-2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16035-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PAI 2, PAI-2, SERPINB2, Monocyte Arg-serpin, PAI2

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Product Information

16035-1-PBS targets SerpinB2/PAI-2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8906

  Product name: Recombinant human SERPINB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-415 aa of BC012609

  Sequence: MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKIYSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Calculated Molecular Weight: 415 aa, 47 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC012609
• Gene Symbol: SERPINB2
• Gene ID (NCBI): 5055
• RRID: AB_2186180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05120
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SerpinB2, a plasminogen activator inhibitor-2 (PAI-2), is a serine protease inhibitor of the serpin superfamily that inactivates tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) (PMID: 7506655). SerpinB2 may have both pro-tumor and anti-tumor effects. High expression of tumor cell-associated SerpinB2 facilitates breast cancer cell survival and metastasis (PMID: 24581498; PMID: 28427146), while SerpinB2 expression by breast cancer cells significantly inhibits metastasis by inhibiting extracellular uPA (PMID: 24644264; PMID: 28346421).