SFI1 Polyclonal antibody, PBS Only

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• SFI1 Polyclonal antibody, PBS Only (13550-1-PBS)

• 

  WB analysis of A549 using 13550-1-AP (same clone as 13550-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

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  WB analysis using 13550-1-AP (same clone as 13550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 13550-1-AP (same clone as 13550-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13550-1-AP (same clone as 13550-1-PBS)

  Immunofluorescent analysis of Hela cells, using SFI1 antibody 13550-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SFI1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 13550-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

hSFI1, KIAA0542, Protein SFI1 homolog

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Product Information

13550-1-PBS targets SFI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4268

  Product name: Recombinant human SFI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 917-1211 aa of BC033613

  Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC

• Full Name: Sfi1 homolog, spindle assembly associated (yeast)
• Calculated Molecular Weight: 1211 aa, 134 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: BC033613
• Gene Symbol: SFI1
• Gene ID (NCBI): 9814
• RRID: AB_10596938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A8K8P3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).