- Featured Product
- KD/KO Validated
SMN (Human-Specific) Monoclonal antibody
SMN (Human-Specific) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
2C6D9
Cat no : 60154-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, Raji cells, HepG2 cells, HEK-293 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human brain tissue, human heart tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
60154-1-Ig targets SMN (Human-Specific) in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | SMN (Human-Specific) fusion protein Ag14333 |
Full Name | survival of motor neuron 2, centromeric |
Calculated Molecular Weight | 282 aa, 30 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC000908 |
Gene Symbol | SMN |
Gene ID (NCBI) | 6607 |
RRID | AB_10639513 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
IHC protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
IF protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
IP protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Chem Biol SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice. | ||