STAT4 Monoclonal antibody

STAT4 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

4A8C9

Cat no : 67568-2-Ig

Synonyms

SLEB11, STAT4



Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67568-2-Ig targets STAT4 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STAT4 fusion protein Ag19545
Full Name signal transducer and activator of transcription 4
Calculated molecular weight 748 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC031212
Gene symbol STAT4
Gene ID (NCBI) 6775
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Protocols

Product Specific Protocols
WB protocol for STAT4 antibody 67568-2-IgDownload protocol
IHC protocol for STAT4 antibody 67568-2-IgDownload protocol
IF protocol for STAT4 antibody 67568-2-IgDownload protocol
FC protocol for STAT4 antibody 67568-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols