STAT4 Monoclonal antibody

STAT4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4A8C9

Cat no : 67568-2-Ig

Synonyms

SLEB11, STAT4

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Validation Data Gallery

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  WB analysis using 67568-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67568-2-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67568-2-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67568-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67568-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67568-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67568-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67568-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67568-2-Ig (STAT4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 67568-2-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human STAT4 (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

67568-2-Ig targets STAT4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT4 fusion protein Ag19545
• Full Name: signal transducer and activator of transcription 4
• Calculated molecular weight: 748 aa, 86 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC031212
• Gene symbol: STAT4
• Gene ID (NCBI): 6775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].