STAT4 Monoclonal antibody
STAT4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
4A8C9
Cat no : 67568-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
67568-2-Ig targets STAT4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | STAT4 fusion protein Ag19545 |
Full Name | signal transducer and activator of transcription 4 |
Calculated molecular weight | 748 aa, 86 kDa |
Observed molecular weight | 86 kDa |
GenBank accession number | BC031212 |
Gene symbol | STAT4 |
Gene ID (NCBI) | 6775 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].
Protocols
Product Specific Protocols | |
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WB protocol for STAT4 antibody 67568-2-Ig | Download protocol |
IHC protocol for STAT4 antibody 67568-2-Ig | Download protocol |
IF protocol for STAT4 antibody 67568-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |