CoraLite®555-conjugated STAT4 Monoclonal antibody

STAT4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4A8C9

Cat no : CL555-67568

Synonyms

SLEB11, STAT4

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL555-67568

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 STAT4 antibody (CL555-67568, Clone: 4A8C9 ) at dilution of 1:200.

• 

  FC experiment of mouse Th1 using CL555-67568

  1X10^6 mouse Th1 were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human STAT4 (CL555-67568, Clone:4A8C9) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67568 targets STAT4 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT4 fusion protein Ag19545
• Full Name: signal transducer and activator of transcription 4
• Calculated Molecular Weight: 748 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC031212
• Gene Symbol: STAT4
• Gene ID (NCBI): 6775
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].