ChromoTek SUMO1-Trap Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  The SUMO1-Trap Agarose (s1ta) was used to immunoprecipitate endogenous SUMO1 and SUMO1ylated proteins from HEK293T cells. Samples of the input lysate (I), and bound (B) fraction were analyzed by Western blot using 10329-1-AP (SUMO1 Polyclonal Antibody). SUMO1 and the commonly SUMO1ylated protein RanGAP1 are highly enriched using the SUMO1-Trap.

The ChromoTek SUMO1-Trap Agarose, Kit for Immunoprecipitation consists of an anti-SUMO-Tag NANOBODY®/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

Cat No. s1tak

Specificity

SUMO1

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Synonyms

Small ubiqutin-related modifier 1, small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, SUMO 1

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Product Information

The ChromoTek SUMO1-Trap Agarose, Kit for Immunoprecipitation consists of an anti-SUMO-Tag NANOBODY®/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

• Description: The SUMO1-Trap Agarose Kit contains SUMO1-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SUMO1 proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to human SUMO1 (Small Ubiquitin-like Modifier 1). The ChromoTek SUMO1-Trap Agarose will immunoprecipitate both monovalent SUMO1 and SUMO1 chains formed by the process of SUMOylation. In addition to human SUMO1, it will also bind SUMO1 homologs from other mammals.
• Binding Capacity: 25 ug of SUMO1 protein (~40 kDa) per 25 uL bead slurry
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: /
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): /
• Compatibility with mass spectrometry: The SUMO1-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3716415
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

• Component: Description
• SUMO1-Trap Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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