ChromoTek SUMO1-Trap Magnetic Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  The SUMO1-Trap Magnetic Agarose (s1tma) was used to immunoprecipitate endogenous SUMO1ylated proteins from a human cell line. Samples of the input lysate (I), non-bound flow-through (F) and bound (B) fraction were analyzed using Western blot. SUMO1ylated proteins are highly enriched using the SUMO1-Trap. Data kindly provided by Prof. Alfred Vertegaal, Leiden University, The Netherlands.

The ChromoTek SUMO1-Trap Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-SUMO1 NANOBODY®/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

Cat No. s1tmak

Specificity

SUMO1

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Synonyms

Small ubiqutin-related modifier 1, small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, SUMO 1

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Product Information

The ChromoTek SUMO1-Trap Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-SUMO1 NANOBODY®/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

• Description: The SUMO1-Trap Magnetic Agarose, Kit for Immunoprecipitation contains SUMO1-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SUMO1 proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to human SUMO1 (Small Ubiquitin-like Modifier 1). The ChromoTek SUMO1-Trap Agarose will immunoprecipitate both monovalent SUMO1 and SUMO1 chains formed by the process of SUMOylation. In addition to human SUMO1, it will also bind SUMO1 homologs from other mammals.
• Binding Capacity: 20 μg of recombinant SUMO1 protein (~40 kDa) per 25 μL bead slurry
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Wash Buffer Compatibility: /
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): /
• Compatibility with mass spectrometry: The SUMO1-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3716416
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

• Component: Description
• SUMO1-Trap Magnetic Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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