ChromoTek SUMO1-Trap Agarose

Validation Data Gallery

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  The SUMO1-Trap Agarose (s1ta) was used to immunoprecipitate endogenous SUMO1 and SUMO1ylated proteins from HEK293T cells. Samples of the input lysate (I), and bound (B) fraction were analyzed by Western blot using 10329-1-AP (SUMO1 Polyclonal Antibody). SUMO1 and the commonly SUMO1ylated protein RanGAP1 are highly enriched using the SUMO1-Trap.

The ChromoTek SUMO1-Trap Agarose consists of an anti-SUMO1 NANOBODY®/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

Cat No. s1ta

Specificity

SUMO1

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Synonyms

Small ubiqutin-related modifier 1, small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, SUMO 1

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Product Information

The ChromoTek SUMO1-Trap Agarose consists of an anti-SUMO1 NANOBODY®/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.

• Description: Immunoprecipitation of endogenous SUMO1 proteins with anti-SUMO1 Nanobody conjugated to agarose beads.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to human SUMO1 (Small Ubiquitin-like Modifier 1). The ChromoTek SUMO1-Trap Agarose will immunoprecipitate both monovalent SUMO1 and SUMO1 chains formed by the process of SUMOylation. In addition to human SUMO1, it will also bind SUMO1 homologs from other mammals.
• Binding Capacity: 25 ug of recombinant SUMO1 protein (~40 kDa) per 25 ul bead slurry
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: /
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): /
• Compatibility with mass spectrometry: The SUMO1-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3716415
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!

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