Validation Data Gallery
Product Information
anti-Spot-Tag® VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
| Description | Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.
• Capture and detection tag without compromises in applications • Higher image resolution • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Strong avidity effect from bivalent form of Spot-VHH • Fulfills highest requirements on antibody validation: structure and function characterized |
| Applications | IF |
| Host | Alpaca |
| Specificity/Target | Spot-Tag® (PDRVRAVSHWSS) |
| Conjugate | ATTO 488 |
| Physical State | Liquid |
| Suggested Dilution | IF: 1:1,000 |
| Type | Nanobody |
| Class | Recombinant |
| RRID | AB_2827569 |
| Affinity (KD) | Dissociation constant KD of 6 nM |
| Spot-Tag® Origin | Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin |
| Cross Reactivity | The Spot-Nanobody shows minimal cross reactivity with beta-catenin. |
| Excitation/ Emission | Excitation range 480 - 510 nm (λabs= 501 nm) Emission range 520 - 560 nm (λfl= 523 nm) |
| Storage Buffer | 1x PBS, 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light. |
| Size | 10 μL; 50 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| eba488_SDS_Spot-Label® ATTO 488 (EN) |
| Datasheet |
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| Protocol Spot-Label ATTO488 |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Moritz (Verified Customer) (09-12-2024) | In HEK293T cells transfected with SFFV::POI-TurboID-SPOT fusion construct, incubated with SPOT atto 488 label (1:1000) the background was very high and no specific signal from SPOT could be observed. Another antibody used as control gave a clear signal of the fusion protein.
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