ChromoTek Spot-Label® Alexa Fluor® 647

Validation Data Gallery

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  SPOT tag related products

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  Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Right: Control staining, no background noise; HeLa cells without pSpot Spot-Tag?-Actin expression were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

Cat No. ebAF647

Specificity

Spot-Tag^®

Applications

IF

Conjugate

Alexa Fluor® 647

Host

Alpaca

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag

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Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

• Description: Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

  • Capture and detection tag without compromises in applications

  • Higher image resolution

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Strong avidity effect from bivalent form of Spot-VHH

  • Fulfills highest requirements on antibody validation: structure and function characterized

• Applications: IF
• Host: Alpaca
• Specificity/Target: Spot-Tag^® (PDRVRAVSHWSS)
• Conjugate: Alexa Fluor® 647
• Physical State: Liquid
• Suggested Dilution: IF: 1:800
• Type: Nanobody; Primary antibody
• Class: Recombinant
• Clonality: Monoclonal
• Clone: CTK0216
• Calculated MW: 30.3 kDa
• RRID: AB_2889376
• Immunogen: Beta-catenin, engineered epitope
• Format: Recombinant alpaca single domain antibody, bivalent
• Affinity (KD): Dissociation constant K_D of 6 nM
• DOL: 1 fluorophore per bivalent Nanobody, site-directed conjugation
• Spot-Tag® Origin: Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
• Cross Reactivity: The Spot-Nanobody shows minimal cross reactivity with beta-catenin.
• Excitation/ Emission: Excitation max: 650 nm, Emission max: 665 nm
• Storage Buffer: PBS, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
• Size: 10 μL; 50 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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