CoraLite®594-conjugated TMP21 Monoclonal antibody

• 

  FC experiment of HeLa using CL594-67876

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMP21 (CL594-67876, Clone:3B11F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

TMP21 Monoclonal Antibody for FC (Intra)

Cat No. CL594-67876

Clone No.3B11F9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Synonyms

TMED10, 21 kDa transmembrane-trafficking protein, 3B11F9, p23, p24 family protein delta-1

---

Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67876 targets TMP21 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7516

  Product name: Recombinant human TMED10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 40-186 aa of BC001825

  Sequence: SRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRV

• Full Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001825
• Gene Symbol: TMED10
• Gene ID (NCBI): 10972
• RRID: AB_2923929
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49755
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.