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CoraLite®594-conjugated TMED10 Monoclonal antibody

TMED10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B11F9

Cat no : CL594-67876

Synonyms

P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated TMED10 Monoclonal antibody (CL594-67876)

    FC experiment of HeLa using CL594-67876

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMED10 (CL594-67876, Clone:3B11F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67876 targets TMED10 in FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMED10 fusion protein Ag7516
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC001825
Gene Symbol TMED10
Gene ID (NCBI) 10972
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for CL594 TMED10 antibody CL594-67876Download protocol
Standard Protocols
Click here to view our Standard Protocols