TMED10 Monoclonal antibody

TMED10 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B11F9

Cat no : 67876-1-Ig

Synonyms

P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21

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Validation Data Gallery

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  WB analysis using 67876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMED10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMED10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67876-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMED10 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, Raji cells, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67876-1-Ig targets TMED10 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMED10 fusion protein Ag7516
• Full Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001825
• Gene Symbol: TMED10
• Gene ID (NCBI): 10972
• RRID: AB_2918633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.