FcZero-rAb™ APC Anti-Human TNF beta Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using APC-FcA98056

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 5 ul APC Anti-Human TNF beta Rabbit RecAb (APC-FcA98056, Clone: 240393C3) and FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD3 (UCHT1) Rabbit IgG Recombinant Antibody (CL488-FcA65570, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

TNF beta FcZero-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. APC-FcA98056

Clone No.240393C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

LTA, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha, TNF β

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Tested Applications

• Positive FC (Intra) detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98056 targets TNF beta in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TNF beta fusion protein Eg0836
• Full Name: lymphotoxin alpha (TNF superfamily, member 1)
• Calculated Molecular Weight: 205 aa, 22 kDa
• GenBank Accession Number: BC034729
• Gene Symbol: TNF beta
• Gene ID (NCBI): 4049
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.