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Tested Applications
| Positive FC (Intra) detected in | Anti-CD3 and CD28 treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-98056 targets TNF beta in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TNF beta fusion protein Eg0836 Predict reactive species |
| Full Name | lymphotoxin alpha (TNF superfamily, member 1) |
| Calculated Molecular Weight | 205 aa, 22 kDa |
| GenBank Accession Number | BC034729 |
| Gene Symbol | TNF beta |
| Gene ID (NCBI) | 4049 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01374 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus TNF beta antibody FITC-98056 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
