TNIK Recombinant antibody

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  WB analysis using 84231-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84231-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84231-4-RR (TNIK antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84231-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84231-4-RR (TNIK antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of K-562 using 84231-4-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84231-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

TNIK Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84231-4-RR

Clone No.241518D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

241518D9, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

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Tested Applications

• Positive WB detected in: T-47D cells, HeLa cells, HEK-293 cells, K-562 cells, COLO 205 cells, Caco-2 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84231-4-RR targets TNIK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TNIK fusion protein Ag34132
• Full Name: TRAF2 and NCK interacting kinase
• Observed Molecular Weight: 155~180 kDa
• GenBank Accession Number: BC150256
• Gene Symbol: TNIK
• Gene ID (NCBI): 23043
• RRID: AB_3671785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UKE5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).