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CoraLite® Plus 488-conjugated TPD52L2 Monoclonal antibody

TPD52L2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B3C6

Cat no : CL488-68077

Synonyms

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CoraLite® Plus 488-conjugated TPD52L2 Monoclonal a (CL488-68077)

    IF Staining of HCT 116 using CL488-68077

    Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using CoraLite® Plus 488 TPD52L2 antibody (CL488-68077, Clone: 3B3C6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TPD52L2 Monoclonal a (CL488-68077)

    FC experiment of HeLa using CL488-68077

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TPD52L2 (CL488-68077, Clone:3B3C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHCT 116 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68077 targets TPD52L2 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPD52L2 fusion protein Ag30863
Full Name tumor protein D52-like 2
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC006804
Gene Symbol TPD52L2
Gene ID (NCBI) 7165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TPD52L2 antibody CL488-68077Download protocol
FC protocol for CL Plus 488 TPD52L2 antibody CL488-68077Download protocol
Standard Protocols
Click here to view our Standard Protocols