TTC30A Polyclonal antibody

• Featured Product
• KD/KO Validated

TTC30A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25352-1-AP

Synonyms

IFT70A, TPR repeat protein 30A, Intraflagellar transport protein 70A

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Validation Data Gallery

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  WB analysis of A431 using 25352-1-AP

  WB result of TTC30A antibody (25352-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC30A transfected A431 cells.

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  WB analysis using 25352-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25352-1-AP (TTC30A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25352-1-AP

  IP result of anti-TTC30A (IP:25352-1-AP, 4ug; Detection:25352-1-AP 1:500) with HEK-293 cells lysate 2600ug.

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  IF Staining of hTERT-RPE1 using 25352-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (25352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse testis, rat testis
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

25352-1-AP targets TTC30A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC30A fusion protein Ag18361
• Full Name: tetratricopeptide repeat domain 30A
• Calculated Molecular Weight: 665 aa, 76 kDa
• Observed Molecular Weight: 72-76 kDa
• GenBank Accession Number: BC042848
• Gene Symbol: TTC30A
• Gene ID (NCBI): 92104
• RRID: AB_2651172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	WB,IF	Biol Open; Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.; Authors - Ryota Takei