Cat no : v5ta
V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to beads.
• No heavy & light antibody chains in downstream applications
• Very low background
• Effective elution with V5-peptide
• Stable under harsh washing conditions
• Efficient immunoprecipitation of V5-tag fusion proteins
|Applications||IP, CoIP, ChIP, RIP, Protein purification|
|Specificity/Target||V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.|
|Binding capacity||15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
SDS sample buffer
0.2 M glycine pH 2.5
|Wash buffer compatibility||2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea|
|Affinity (KD)||Dissociation constant KD of 40 nM|
|Compatibility with mass spectrometry||The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
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Metabolic novelty originating from horizontal gene transfer is essential for leaf beetle survival