ChromoTek V5-Trap™ Agarose Kit

V5-Trap™ Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : v5tak

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

V5-Trap™ Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Coupled VHHRecombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 6 µg of recombinant V5-tagged protein (~30 kDa)
Elution bufferV5-peptide
SDS sample buffer
0.1 M glycine pH 2.0
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
V5-Trap™ Agarose 20 reactions (500 µL)
Lysis buffer Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution buffer Dilution of cell lysate