ChromoTek V5-Trap® Agarose Kit

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : v5tak

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

DescriptionThe V5-Trap Agarose Kit contains V5-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Effective elution with V5-peptide

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2868497
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
V5-Trap™ Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate