ChromoTek V5-Trap® Agarose, Kit for Immunoprecipitation

Validation Data Gallery

• 

  V5-Trap® Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-Through, B: Bound.

• 

  V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.

• 

  V5-Trap® Agarose for pull-down. The V5-tag is N-terminal. I: Input, FT: Flow-Through, B: Bound.

• 

  IP from different cell lysates without V5-tagged proteins => The V5-Trap® has very low background.

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

Cat No. v5tak

Publications(1)

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

---

Product Information

V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

• Description: The V5-Trap Agarose Kit contains V5-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Effective elution with V5-peptide

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP, Protein purification
• Specificity/Target: V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
• Binding capacity: 15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: V5-peptide; SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 40 nM
• Compatibility with mass spectrometry: The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2868497
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

• Component: Description
• V5-Trap™ Agarose: 20 reactions (500 µL)
• Lysis buffer: Optimized for cytoplasmatic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate

---

Publications

Application	Title
	PLoS One; PSMD11 modulates circadian clock function through PER and CRY nuclear translocation; Authors - Sibel Cal-Kayitmazbatir