ChromoTek V5-Trap® Magnetic Particles M-270

The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat no : v5td

Synonyms

Magnetic Particles M-270, V5-Trap Magnetic Particles M-270, V5 tag, V5-tag, V5-Trap®, V5-Trap® Magnetic Particles M-270, v5td, v5td-10, v5td-100, v5td-20, v5td-200, v5td-400



Product Information

The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

DescriptionImmunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to magnetic particles. V5-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.

• No heavy & light antibody chains in downstream applications

• Very low background

• Effective elution with V5-peptide

• Stable under harsh washing conditions

• Efficient immunoprecipitation of V5-tag fusion proteins

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity0.62-1.25 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 150 mM NaCl, 10 mM TCEP, 10 mM DTT,  50 mM Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2892994
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!