YES1-Specific Polyclonal antibody, PBS Only

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• YES1-Specific Polyclonal antibody, PBS Only (20243-1-PBS)

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  WB analysis using 20243-1-AP (same clone as 20243-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20243-1-AP (YES1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20243-1-AP (same clone as 20243-1-PBS)

  WB result of YES1-Specific antibody (20243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 20243-1-AP (same clone as 20243-1-PBS)

  IP result of anti-YES1-Specific (IP:20243-1-AP, 4ug; Detection:20243-1-AP 1:500) with A549 cells lysate 1400 ug. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20243-1-AP (same clone as 20243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20243-1-AP (same clone as 20243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 20243-1-AP (same clone as 20243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 20243-1-AP (same clone as 20243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

YES1-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 20243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

YES1, c yes, EC:2.7.10.2, HsT441, P61 YES

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Product Information

20243-1-PBS targets YES1-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: NM_005433
• Gene Symbol: YES1
• Gene ID (NCBI): 7525
• RRID: AB_10697656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07947
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.