ChromoTek anti-Rabbit IgG / anti-Mouse IgG VHH Agarose for Immunoprecipitation

Validation Data Gallery

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  Co-IP of BAX and BAK using anti-BAX IgGs from different vendors using anti-ms / anti-rb IgG VHH Agarose (mrIGa). Tested IgGs include such of rabbit and mouse origin with IgG1 and IgG2b subtypes. 5 µg of respective IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of Input (IN) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western Blot analysis BAX was detected using a polyclonal rb IgG (PTG: 50599-2-Ig) (1:2000) labeled using the FlexAble HRP Antibody Labeling Kit for rabbit IgG. This allows a clean detection of BAX, without interfering signals of IgG heavy and light chains derived from the spiked rabbit antibodies. The presence of BAK co-precipitated with BAX was tested using a polyclonal  rb IgG (PTG 29552-1-AP) 1:2000, labeled with FlexAble HRP. While all IgGs allowed for efficient IP of BAX, BAK was absent in the case of the primary antibody from vendor D, possibly due to the BAX:BAK interaction interface being masked by this particular IgG.

anti-Rabbit / anti-Mouse IgG IP Beads is an affinity resin for IP of all subtypes of Rabbit and Mouse IgG. It consists of rabbit and mouse IgG specific VHHs (Nanobodies) coupled to Agarose beads.

Cat No. mrIGa

Reactivity

Rabbit IgG / Mouse IgG

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Mouse IP, Rabbit IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

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Product Information

anti-Rabbit / anti-Mouse IgG IP Beads is an affinity resin for IP of all subtypes of Rabbit and Mouse IgG. It consists of rabbit and mouse IgG specific VHHs (Nanobodies) coupled to Agarose beads.

• Description: Ready to use VHH coupled Agarose Beads for Immunoprecipitation of all subtypes of Rabbit and Mouse IgG. ;

  • Fast, reliable and efficient one-step Immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • Specific to all Rabbit and all Mouse IgG subtypes;

  • Low background

• Applications: IP, Co-IP
• Binding capacity: /
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS Sample Buffer
• Wash buffer compatibility: /
• Type: Nanobody
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity (KD): /
• Immunogen: Rabbit IgG / Mouse IgG
• RRID: AB_3697100
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp
• Size: 1 ml; 2 ml; 5 ml

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