mCherry Recombinant antibody

• 

  WB analysis using 81202-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81202-2-RR (mCherry antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Transfected HEK-293 using 81202-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using mCherry antibody (81202-2-RR, Clone: 241830F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Transfected HEK-293T using 81202-2-RR

  1x10^6 HEK-293T cells or mCherry Transfected HEK-293T cells were intracellularly stained with 0.25 ug mCherry Recombinant antibody (81202-2-RR, Clone:241830F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 81202-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81202-2-RR against mCherry were performed. The affinity constant is 0.205 nM.

mCherry Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 81202-2-RR

Clone No.241830F8

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241830F8

---

Tested Applications

• Positive WB detected in: Transfected HEK-293T cells
• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81202-2-RR targets mCherry in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: mCherry fusion protein Ag25320
• Full Name: mCherry
• Calculated Molecular Weight: 27 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogeneous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitating visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against mCherry.