CoraLite® Plus 488-conjugated smooth muscle actin specific Recombinant antibody
smooth muscle actin specific Recombinant Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig, chicken
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5H7
Cat no : CL488-80008
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse heart tissue |
Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-80008 targets smooth muscle actin specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.
Tested Reactivity | human, mouse, rat, pig, chicken |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | actin, alpha 2, smooth muscle, aorta |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_001613 |
Gene Symbol | Alpha smooth muscle actin |
Gene ID (NCBI) | 59 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 smooth muscle actin specific antibody CL488-80008 | Download protocol |
FC protocol for CL Plus 488 smooth muscle actin specific antibody CL488-80008 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |