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CoraLite® Plus 488-conjugated Albumin Monoclonal antibody (CL488-66051)

CoraLite® Plus 488-conjugated Albumin Monoclonal antibody

Name: CoraLite® Plus 488-conjugated Albumin Monoclonal antibody, 100ul
Brand: Proteintech
SKU: CL488-66051-100UL

Albumin Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66051

Clone No. 4A1C11

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

IF/ICC, FC (Intra)

Synonyms

ALB, 4A1C11, HSA, Serum albumin

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite® Plus 488

Unconjugated CoraLite® Plus 488 CoraLite® Plus 750 CoraLite®594

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Filter: AllIF/ICCFC (Intra)

IF Staining of HepG2 using CL488-66051
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Albumin antibody (CL488-66051, Clone: 4A1C11 ) at dilution of 1:400.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HepG2 using CL488-66051
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Albumin (CL488-66051, Clone:4A1C11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Albumin in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66051 targets Albumin in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Reactivity	human, rat, pig
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9885 Product name: Recombinant human Albumin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 256-609 aa of BC034023 Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL Predict reactive species
Full Name	albumin
Calculated Molecular Weight	609 aa, 69 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC034023
Gene Symbol	Albumin
Gene ID (NCBI)	213
RRID	AB_2883241
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	493 nm / 522 nm
Excitation Laser	Blue laser (488 nm)
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P02768
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Albumin antibody CL488-66051	Download protocol
IF protocol for CL Plus 488 Albumin antibody CL488-66051	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

CL488-66051 is an Albumin Monoclonal antibody (4A1C11) conjugated with CoraLite® Plus 488. It has 70 total citations published across journals including Cell Metabolism, Nature Communications, FASEB Journal, EMBO Journal, Hepatology, and Journal of Clinical Investigation. Research fields span liver disease (NAFLD/NASH, fibrosis, liver cancer), lipid metabolism, extracellular vesicles, ferroptosis, diabetic retinopathy, neuroscience, and stem cell/organoid research.