Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse heart tissue, mouse thymus tissue, mouse spleen tissue. mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 815 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
HRP-60004 targets GAPDH in WB, IHC, IF, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Reactivity | human, mouse, rat, zebrafish |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, chicken, hamster, sheep, honey bees |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2737588 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the molecular weight of GAPDH?
The molecular weight of GAPDH is 36 kD.
What is GAPDH?
GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping"
protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic
production of ATP and pyruvate (PMID: 21895736).
What is the function of GAPDH?
GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even
critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH
is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting
as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in
transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407,
11035021).
Where in the cell is GAPDH localized?
GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus
(PMIDs: 21895736, 12829261).
Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear
functions?
Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is
useful as a control for Western blots and RT-PCR.
What are the known protein-protein interactions of GAPDH?
GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of
these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).
Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP GAPDH antibody HRP-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Biotechnol Direct targeting of amplified gene loci for proapoptotic anticancer therapy. | ||
Cell Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity. | ||
Cell Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ann-Kathrin (Verified Customer) (11-12-2025) | The conjugated GAPDH Antibody work really well, especially with semidry transfer. After blocking the blot for 2h in 7% skim milk/TBST and incubating for 1h with the antibody in /% skim milk/TBST the blot is ready to detect. Unsing this antibody saves time and it really cost efficient.
|
FH JIN-FENG (Verified Customer) (10-01-2025) | GAPDH-HRP works perfectly on my blot. I used it as my internal control and it performed very specific signal on the blot.
 |
FH STEVE (Verified Customer) (08-22-2025) | I really liked this antibody coupled with HRP, specific and reliable.
|
FH Moritz (Verified Customer) (09-12-2024) | The antibody worked very well at a dilution of 1:10k in WB using HEL293T cell lysate as a loading control.
 |
FH Julia (Verified Customer) (08-07-2024) | Really good antibody with specific band at the correct size. Conjugation with HRP allows quickworks great to get results quickly.
 |
FH T (Verified Customer) (03-20-2024) | Worked great with multiple tissue types. Highly recommend!
|
FH Tsimafei (Verified Customer) (07-15-2022) | 30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.
 |
FH Hala (Verified Customer) (02-25-2022) | it works very good but i got an aspecific band around 30KDa
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FH Laura (Verified Customer) (01-10-2020) | Works well
|
FH Benjamin (Verified Customer) (01-07-2020) | Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.
|
FH Kathryn (Verified Customer) (12-10-2019) | Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.
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