|Positive IHC detected in||human tonsillitis tissue, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human skin cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Immunohistochemistry (IHC)||IHC : 1:8000-1:20000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 27309-1-AP immunogen is Fusion Protein KI67 protein expressed in E. coli.
|Cited Reactivity||canine, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KI67 fusion protein Ag26266|
|Full Name||antigen identified by monoclonal antibody Ki-67|
|Calculated molecular weight||359 kDa|
|Observed molecular weight|
|GenBank accession number||NM_002417|
|Gene ID (NCBI)||4288|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.
β-Sitosterol and Gemcitabine Exhibit Synergistic Anti-pancreatic Cancer Activity by Modulating Apoptosis and Inhibiting Epithelial-Mesenchymal Transition by Deactivating Akt/GSK-3β Signaling.
The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.
Cell Death Dis
NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6.
In Vitro Cell Dev Biol Anim
miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A.
The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yan (Verified Customer) (02-18-2020)
1:100 work for cell.