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iNOS Antibody 76 Publications

Rabbit Polyclonal| Catalog number: 18985-1-AP

Featured Product KD/KO validated

2 customer reviews

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

iNOS Antibody WB HeLa cells 18985-1-AP

WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells

iNOS Antibody IHC human liver tissue 18985-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18985-1-AP( iNOS Antibody) at dilution of 1:200 (under 10x lens).

iNOS Antibody IHC human liver tissue 18985-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18985-1-AP( iNOS Antibody) at dilution of 1:200 (under 40x lens).

iNOS Antibody WB HeLa cells 18985-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

iNOS Antibody IF HeLa cells 18985-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 18985-1-AP(iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, chicken
Positive WB detected in:
HeLa cells
Positive IHC detected in:
human liver tissue, mouse liver tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
nitric oxide synthase 2, inducible
Calculated molecular weight:
131 kDa
Observed molecular weight:
110-130 kDa, 65-70 kDa
GenBank accession number:
NM_000625
Gene ID (NCBI):
4843
Gene symbol
NOS2
Synonyms
HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

Background

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

iNOS Rabbit Polyclonal antibody

Prasanth (Verified Customer), 08-19-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Mouse Fibroblast Cells
Lot Number
This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

iNOS Rabbit Polyclonal antibody

Elena (Verified Customer), 08-14-2019
Applications
Western Blot,
No image submitted with review

No image submitted with review

Lot Number
We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

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