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iNOS Antibody 48 Publications

Rabbit Polyclonal| Catalog number: 18985-1-AP

Featured Product KD/KO validated

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Con: 40 μg/150 μl

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Tested Applications

Applications:
IHC, IF, ELISA
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Images See all

iNOS Antibody WB HeLa cells 18985-1-AP

WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells

iNOS Antibody IHC human liver tissue 18985-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 18985-1-AP( iNOS Antibody) at dilution of 1:200 (under 10x lens).

iNOS Antibody IHC human liver tissue 18985-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 18985-1-AP( iNOS Antibody) at dilution of 1:200 (under 40x lens).

iNOS Antibody IF HeLa cells 18985-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 18985-1-AP(iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, broiler, chicken
Positive IHC detected in:
human liver tissue, mouse liver tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
nitric oxide synthase 2, inducible
Calculated molecular weight:
131 kDa
Observed molecular weight:
130-140 kDa
GenBank accession number:
NM_000625
Gene ID (NCBI):
4843
Gene symbol
NOS2
Synonyms
HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

Background

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.


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