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iNOS Polyclonal antibody

iNOS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18985-1-AP

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

Validation Data Gallery

  • WB analysis of HeLa using 18985-1-AP

    WB analysis of HeLa using 18985-1-AP

    WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells.

  • WB analysis using 18985-1-AP

    WB analysis using 18985-1-AP

    Mouse liver lysates were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 18985-1-AP

    IHC staining of human liver using 18985-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18985-1-AP (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 18985-1-AP

    IHC staining of human liver using 18985-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18985-1-AP (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 18985-1-AP

    IF Staining of HeLa using 18985-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18985-1-AP (iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18985-1-AP targets iNOS in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 2, inducible
Calculated molecular weight 131 kDa
Observed molecular weight 110-130 kDa, 65-70 kDa
GenBank accession numberNM_000625
Gene symbol NOS2
Gene ID (NCBI) 4843
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Protocols

Product Specific Protocols
WB protocol for iNOS antibody 18985-1-APDownload protocol
IHC protocol for iNOS antibody 18985-1-APDownload protocol
IF protocol for iNOS antibody 18985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biol Trace Elem Res

Mechanism Underlying the Protective Effect of Selenium on NO-Induced Oxidative Damage in Bovine Mammary Epithelial Cells.

Authors - Yongmei Guo
View Article
ratWB,IHC

Iran J Basic Med Sci

Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.

Authors - Yeying Wang
View Article
mouseWB

Oxid Med Cell Longev

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice.

Authors - Yingjuan Ma
View Article
ratWB

Transl Neurosci

Fangchinoline Ameliorates the Expressions of Angiogenic Molecule in Cerebral Ischemia Induced Neuronal Degeneration in Neonatal Rats.

Authors - Han Daicheng
View Article
mouseIHC

Sci Rep

Ghrelin protects against contact dermatitis and psoriasiform skin inflammation by antagonizing TNF-α/NF-κB signaling pathways.

Authors - Ruize Qu
View Article
mouseWB

Oxid Med Cell Longev

Role of Antioxidants in the Protection from Aging-Related Diseases.

Authors - Daria Maria Monti
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblast Cells
FH

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

  • Applications: Western Blot,