|Positive WB detected in||mouse liver tissue, HeLa cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18985-1-AP targets iNOS in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Full Name||nitric oxide synthase 2, inducible|
|Calculated molecular weight||131 kDa|
|Observed molecular weight||110-130 kDa, 65-70 kDa|
|GenBank accession number||NM_000625|
|Gene ID (NCBI)||4843|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Prasanth (Verified Customer) (08-19-2019)
This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.
Elena (Verified Customer) (08-14-2019)
We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.