Beta Tubulin Monoclonal antibody

Beta Tubulin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, nematode, pig, zebrafish and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D4A4

Cat no : 66240-1-Ig

Synonyms

beta tubulin, MC1R, TUBB, Tubulin-beta



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human breast cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66240-1-Ig targets Beta Tubulin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, nematode, pig, zebrafish samples.

Tested Reactivity human, mouse, rat, nematode, pig, zebrafish
Cited Reactivity human, mouse, rabbit, rat, swine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0117
Full Name tubulin, beta 3
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000748
Gene symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.

 

What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?

The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).

 

How to use beta-tubulin as a loading control

Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

 

What drugs can influence beta-tubulin and organization of microtubules?

Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.

 

Is beta-tubulin post-translationally modified?

Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).

Protocols

Product Specific Protocols
WB protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IHC protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IF protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IP protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Genet

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Authors - Yan-Wei Sha
ratWB

Transpl Immunol

PMID: 30682409

Authors - Jin Deng
humanWB

J Biol Chem

Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.

Authors - Zhoufei Hei
mouseWB

Neurobiol Dis

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Authors - Qi Wang
humanWB

FASEB J

Resveratrol targets TyrRS acetylation to protect against radiation-induced damage.

Authors - Piaoyang Gao
humanWB

Front Oncol

Nuclear Receptor Coactivator 2 Promotes Human Breast Cancer Cell Growth by Positively Regulating the MAPK/ERK Pathway.

Authors - Mengjiao Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yu Siong (Verified Customer) (08-18-2020)

Western blot of 4 bands: 4 different treatments.

  • Applications: Western Blot,Cell culture
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: primary porcine brain endothelial cells
Beta Tubulin Antibody Western Blot,Cell culture validation (1:5000 dilution) in primary porcine brain endothelial cells (Cat no:66240-1-Ig)
FH

Kathryn (Verified Customer) (12-10-2019)

Used 1:20000 in 5% BSA on a small amount of neonatal mouse ovary. Very clean and specific, and great that it works at such a low concentration.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: mouse ovary
Beta Tubulin Antibody Western Blot, validation (1:20000 dilution) in mouse ovary (Cat no:66240-1-Ig)
FH

Sara (Verified Customer) (10-09-2019)

This antibody works great in WB. We use it as loading control in whole cell lysates (mice B cell line), 1:10.000 O/N at 4°C.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10.000
  • Cell Tissue Type: mice, B lymphocytes
FH

VLADIMIR (Verified Customer) (09-25-2019)

B-tubulin IgG2a were used to prepare Fab' conjugated with Alexa 647-maleimide

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HeLa
FH

Jeffery (Verified Customer) (08-01-2019)

Very clean antibody. Works exceptionally well. Runs right at the 50kd marker.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20,000
  • Cell Tissue Type: Ins-1 rat beta cells
Beta Tubulin Antibody Western Blot, validation (1:20,000 dilution) in Ins-1 rat beta cells (Cat no:66240-1-Ig)
FH

Shashirekha (Verified Customer) (05-09-2019)

Works great!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: NSC-34
Beta Tubulin Antibody Western Blot, validation (1:5000 dilution) in NSC-34 (Cat no:66240-1-Ig)
FH

Zunair (Verified Customer) (12-03-2018)

Ordered this antibody as a housekeeping loading control. Worked great! Used the Licor Odyssey Western Blotting system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: 786-O [786-0] (ATCC® CRL-1932™)
Beta Tubulin Antibody Western Blot, validation (1:5000 dilution) in 786-O [786-0] (ATCC® CRL-1932™) (Cat no:66240-1-Ig)