"Beta Tubulin Antibodies" Comparison
View side-by-side comparison of Beta Tubulin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells, ROS1728 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human breast cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66240-1-Ig targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, nematode samples.
| Tested Reactivity | human, mouse, rat, pig, zebrafish, nematode |
| Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, zebrafish, sheep, yeast |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0117 Product name: Recombinant human Tubulin-beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-259 aa of BC000748 Sequence: LERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVP Predict reactive species |
| Full Name | tubulin, beta 3 |
| Calculated Molecular Weight | 450 aa, 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC000748 |
| Gene Symbol | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2881629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13509 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.
What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?
The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).
How to use beta-tubulin as a loading control
Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.
What drugs can influence beta-tubulin and organization of microtubules?
Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.
Is beta-tubulin post-translationally modified?
Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IHC protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IP protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| WB protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Tubulin antibody (Cat# 66240-1-Ig) has accumulated 812 citations in high-impact journals including Nature, Science, Cell, Nature Communications, Nature Cell Biology, Signal Transduction and Targeted Therapy, Advanced Science, Cell Death & Differentiation, Molecular Cell, and Autophagy. Research fields span oncology, cell biology, immunology, neuroscience, cardiology, metabolism, microbiology, gene editing, and nanomedicine, with Western blot as the predominant application.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation | ||
Signal Transduct Target Ther O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N6-methyladenosine-dependent manner | ||
Reviews
What customers say
All 15 reviews are 5-star ratings, with the vast majority using this antibody for Western Blot as a loading control. Reviewers consistently report a clean, strong, and specific single band at ~50–55 kDa across diverse cell types including HEK293T, mESCs, prostate cancer lines, mouse ovary tissue, and rat beta cells. Many highlight its performance at high dilutions (up to 1:20,000), calling it reliable and their standard housekeeping control. One user also successfully applied it for immunofluorescence after Fab' conjugation. Overall, users describe it as exceptionally well-performing with minimal background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Audrey (Verified Customer) (04-10-2026) | Works well for WB
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Aditya (Verified Customer) (11-01-2024) | great with flourescence, our standard housekeeping control
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P (Verified Customer) (09-23-2024) | Excellent
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Moritz (Verified Customer) (09-12-2024) | The antibody worked very well and gave a very strong signal (can be diluted more). Used it in HEK293T cell lysate analysed with WB.
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Tsimafei (Verified Customer) (08-04-2024) | Excellent antibody! The membrane was incubated for 1h at RT
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Andrea (Verified Customer) (01-11-2023) | Antibody were incubated for 2h at room temperature. Provide a specific tubulin signal
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Emma (Verified Customer) (11-29-2021) | Very clean antibody and runs at the right size by western blot.
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Raul (Verified Customer) (08-20-2021) | Very good antibody producing a single strong band at ~55kDa
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Yu Siong (Verified Customer) (08-18-2020) | Western blot of 4 bands: 4 different treatments.
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Kathryn (Verified Customer) (12-10-2019) | Used 1:20000 in 5% BSA on a small amount of neonatal mouse ovary. Very clean and specific, and great that it works at such a low concentration.
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Sara (Verified Customer) (10-09-2019) | This antibody works great in WB. We use it as loading control in whole cell lysates (mice B cell line), 1:10.000 O/N at 4°C.
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VLADIMIR (Verified Customer) (09-25-2019) | B-tubulin IgG2a were used to prepare Fab' conjugated with Alexa 647-maleimide
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Jeffery (Verified Customer) (08-01-2019) | Very clean antibody. Works exceptionally well. Runs right at the 50kd marker.
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Shashirekha (Verified Customer) (05-09-2019) | Works great!
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Zunair (Verified Customer) (12-03-2018) |
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