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CoraLite® Plus 488-conjugated Beta Catenin Monoclonal antibody

Beta Catenin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8A1

Cat no : CL488-66379

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β-Catenin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated Beta Catenin Monoclo (CL488-66379)

    IF Staining of MCF-7 using CL488-66379

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Beta Catenin antibody (CL488-66379, Clone: 1B8A1 ) at dilution of 1:200, Alpha Tubulin antibody (11224-1-AP, red).

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated Beta Catenin Monoclo (CL488-66379)

    FC experiment of MCF-7 using CL488-66379

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Catenin (CL488-66379, Clone:1B8A1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66379 targets Beta Catenin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight 781 aa, 86 kDa
GenBank Accession NumberNM_001904
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
RRIDAB_2883323
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Beta Catenin antibody CL488-66379Download protocol
FC protocol for CL Plus 488 Beta Catenin antibody CL488-66379Download protocol
Standard Protocols
Click here to view our Standard Protocols