CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

FITC Fluorescent Dye

CloneNo.

ABL-93

Kat-Nr. : FITC-65052

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

---

• 

  FC experiment of NIH/3T3 using FITC-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 2 ul FITC Anti-Mouse CD107b (FITC-65052, Clone:ABL-93) (red), or 2 ul Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65052 bindet in FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 2
• GenBank-Zugangsnummer: BC138718
• Gene symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).