CoraLite®488 Anti-Mouse CD107b (ABL-93)

CD107b Monoclonal Antibody for IF,FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF,FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

ABL-93

Cat no : CL488-65052

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3



Tested Applications

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 65052-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65052 targets CD107b in IF,FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
Full Name lysosomal-associated membrane protein 2
GenBank accession numberBC138718
Gene symbol Lamp2
Gene ID (NCBI) 16784
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for CL488 CD107b antibody CL488-65052Download protocol
FC protocol for CL488 CD107b antibody CL488-65052Download protocol
Standard Protocols
Click here to view our Standard Protocols