Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-65052 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Glycoproteins purified from BALB/c mouse embryo 3T3 cell line Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| GenBank Accession Number | BC138718 |
| Gene Symbol | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| RRID | AB_2919925 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P17047 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 CD107b / LAMP2 antibody CL594-65052 | Download protocol |
| IF protocol for CL594 CD107b / LAMP2 antibody CL594-65052 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is Anti-Mouse CD107b / LAMP2 (ABL-93), catalog number CL594-65052. It has 1 citation, published in Molecular Neurobiology (2025). The cited study investigated OSBP's role in neural damage repair by regulating lysosome transport under oxidative stress, using this antibody for immunofluorescence in mouse samples. The research field encompasses neurobiology and lysosomal biology.
| Species | Application | Title |
|---|---|---|
Mol Neurobiol OSBP Participates in Neural Damage Repair by Regulating Lysosome Transport Under Oxidative Stress |
