Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

ABL-93

Cat no : 65052-1-Ig

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

---

Validation Data Gallery

• 

  IF Staining of NIH/3T3 using 65052-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65052-1-Ig (CD107b antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  FC experiment of NIH/3T3 using 65052-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.50 ug Anti-Mouse CD107b (65052-1-Ig, Clone: ABL-93) (red) or 0.50 ug isotype control antibody (blue), and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

---

Tested Applications

• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Product Information

65052-1-Ig targets CD107b / LAMP2 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
• Full Name: lysosomal-associated membrane protein 2
• GenBank accession number: BC138718
• Gene symbol: Lamp2
• Gene ID (NCBI): 16784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).