Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65052-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Glycoproteins purified from BALB/c mouse embryo 3T3 cell line Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| GenBank Accession Number | BC138718 |
| Gene Symbol | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| RRID | AB_2881468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P17047 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD107b / LAMP2 antibody 65052-1-Ig | Download protocol |
| IF protocol for CD107b / LAMP2 antibody 65052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD107b/LAMP2 (ABL-93), catalog #65052-1-Ig, has 3 citations published in ACS Nano, Cell Reports in Medicine, and Metabolism. The research fields span atherosclerosis nanoparticle therapy, NLRP3 inflammasome activation in diabetic wound healing, and MASLD involving hepatocyte senescence and lysosomal dysfunction. All cited applications are IF (immunofluorescence) in mouse samples.
| Species | Application | Title |
|---|---|---|
ACS Nano Autophagy-Inducing and cfDNA-Scavenging Nanoparticles for Synergistic Atherosclerosis Therapy. | ||
Cell Rep Med Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing | ||
Metabolism ASGR1 exacerbates MASLD by inducing hepatocyte senescence via lysosomal dysfunction and HIF-1α stabilization. |
