Anti-Mouse CD107b / LAMP2 (ABL-93)

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  IF Staining of NIH/3T3 using 65052-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65052-1-Ig (CD107b antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of NIH/3T3 using 65052-1-Ig

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b / LAMP2 (65052-1-Ig, Clone: ABL-93) and FITC anti-Rat IgG2a Antibody (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65052-1-Ig

Clone No.ABL-93

Publications(1)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Synonyms

ABL93, CD107b, Lamp2, ABL-93, Lamp 2

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65052-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Glycoproteins purified from BALB/c mouse embryo 3T3 cell line

• Full Name: lysosomal-associated membrane protein 2
• GenBank Accession Number: BC138718
• Gene Symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• RRID: AB_2881468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P17047
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv