Anti-Mouse CD107b / LAMP2 (ABL-93)

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  IF Staining of NIH/3T3 using 65052-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65052-1-Ig (CD107b antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of NIH/3T3 using 65052-1-Ig

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b / LAMP2 (65052-1-Ig, Clone: ABL-93) and FITC anti-Rat IgG2a Antibody (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65052-1-Ig

Clone No.ABL-93

Publications(1)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC (Intra)

Synonyms

CD107b, Lamp2, Mac3, Lamp 2, ABL-93

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65052-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
• Full Name: lysosomal-associated membrane protein 2
• GenBank Accession Number: BC138718
• Gene Symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• RRID: AB_2881468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P17047
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Publications

Species	Application	Title
mouse	IF	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv