CD83 Monoklonaler Antikörper

CD83 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

HB15e

Kat-Nr. : 65171-1-Ig

Synonyme

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

---

• 

  FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

  1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 0.5 ug Anti-Human CD83 (65171-1-Ig, Clone:HB15e) and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (green), or stained with 0.5 ug mouse IgG1 isotype control and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (black). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Human monocytes-derived dendritic cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65171-1-Ig bindet in FC CD83 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane CD83-transfizierte Cos-Zellen
• Vollständiger Name: CD83 molecule
• Berechnetes Molekulargewicht: 205 aa, 23 kDa
• GenBank-Zugangsnummer: BC030830
• Gene symbol: CD83
• Gene ID (NCBI): 9308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).