Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67634-1-Ig targets GSTM3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7494 Product name: Recombinant human GSTM3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-225 aa of BC000088 Sequence: MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC Predict reactive species |
| Full Name | glutathione S-transferase mu 3 (brain) |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC000088 |
| Gene Symbol | GSTM3 |
| Gene ID (NCBI) | 2947 |
| RRID | AB_2882835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21266 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTM3 antibody 67634-1-Ig | Download protocol |
| IHC protocol for GSTM3 antibody 67634-1-Ig | Download protocol |
| WB protocol for GSTM3 antibody 67634-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTM3 Monoclonal antibody (1E2B8), catalog number 67634-1-Ig, has a total of 1 citation. The citation appears in Reproductive Biology and Endocrinology (IF 5.7), published in July 2022. The study investigated a loss-of-function variant in SSFA2 causing male infertility with globozoospermia and failed oocyte activation. The antibody was used in IF and CoIP applications on human samples, placing the research field within reproductive biology and male infertility.
| Species | Application | Title |
|---|---|---|
Reprod Biol Endocrinol A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation. |
