CoraLite®488-conjugated CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1A5A3

Cat no : CL488-66446

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66446-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66446 targets CBX3 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CBX3 fusion protein Ag19112
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
GenBank accession numberBC000954
Gene symbol CBX3
Gene ID (NCBI) 11335
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Protocols

Product Specific Protocols
IF protocol for CL488 CBX3 antibody CL488-66446Download protocol
FC protocol for CL488 CBX3 antibody CL488-66446Download protocol
Standard Protocols
Click here to view our Standard Protocols