FITC Anti-Mouse CD107b / LAMP2 (ABL-93)

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  FC experiment of NIH/3T3 using FITC-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 2 ul FITC Anti-Mouse CD107b (FITC-65052, Clone:ABL-93) (red), or 2 ul Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD107b / LAMP2 Monoclonal Antibody for FC (Intra)

Cat No. FITC-65052

Clone No.ABL-93

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Tested Applications

• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 2 ul per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65052 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
• Full Name: lysosomal-associated membrane protein 2
• GenBank Accession Number: BC138718
• Gene Symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• RRID: AB_2883742
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P17047
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).