Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.06 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65102 targets GITR/TNFRSF18 in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse CD25+ CD4+ T cells Predict reactive species |
| Full Name | tumor necrosis factor receptor superfamily, member 18 |
| GenBank Accession Number | BC146517 |
| Gene Symbol | CD357 |
| Gene ID (NCBI) | 21936 |
| RRID | AB_2883775 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | O35714 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).
Publications
What published studies show
FITC-65102 is an Anti-Mouse GITR/TNFRSF18 (DTA-1) monoclonal antibody with a total of 1 citation. The citation appears in Advanced Healthcare Materials (Impact Factor: 11), published in 2025. The research field is tumor immunotherapy, specifically involving engineered hollow nanocomplexes combining photothermal and antioxidant strategies for targeted Treg cell depletion and immune activation in mice, using IF as the application.
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy |
