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EIF4E Monoclonal antibody
EIF4E Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3C6B9
Cat no : 66655-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, U2OS cells, LNCaP cells, Jurkat cells, HEK-293 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
66655-1-Ig targets EIF4E in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF4E fusion protein Ag27191 |
Full Name | eukaryotic translation initiation factor 4E |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 25 kDa |
GenBank accession number | BC012611 |
Gene symbol | EIF4E |
Gene ID (NCBI) | 1977 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance.
Protocols
Product Specific Protocols | |
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WB protocol for EIF4E antibody 66655-1-Ig | Download protocol |
IHC protocol for EIF4E antibody 66655-1-Ig | Download protocol |
IF protocol for EIF4E antibody 66655-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Immunother Cancer Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. | ||
Neurosci Bull miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice.
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