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EIF4E Monoclonal antibody

EIF4E Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3C6B9

Cat no : 66655-1-Ig

Synonyms

CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein



Tested Applications

Positive WB detected inHeLa cells, U2OS cells, LNCaP cells, Jurkat cells, HEK-293 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66655-1-Ig targets EIF4E in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4E fusion protein Ag27191
Full Name eukaryotic translation initiation factor 4E
Calculated molecular weight 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012611
Gene symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance.

Protocols

Product Specific Protocols
WB protocol for EIF4E antibody 66655-1-IgDownload protocol
IHC protocol for EIF4E antibody 66655-1-IgDownload protocol
IF protocol for EIF4E antibody 66655-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunother Cancer

Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells.

Authors - Shasha Zhao
mouseWB,IHC

Neurosci Bull

miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice.

Authors - Xiao Ke
  • KD Validated