EMT Expanded Antibody Kit




Target Dependent


Target Dependent



Cat no : PK30015

Product Information

The EMT Expanded Antibody Kit provides a cost-effective tool for studying key cell markers, transcription factors, and ECM degraders invovled in the epithelial to mesenchymal transition (EMT). Perfect for cancer and developmental biology researchers starting a new project, screening multiple prospective targets, or those who simply require less volume. 

The EMT Expanded Antibody Kit contains antibodies against 10 key protein targets that play critical roles in epithelial to mesenchymal transition.

Antigen Catalog No. Host, clonality Tested Reactivity Applications Volume
E-cadherin 60335-1-Ig             Mouse monoclonal H, R, Pg             WB, IHC, IF, FC, ELISA              20 uL
N-cadherin             66219-1-Ig             Mouse monoclonal             H, M, R, Pg, Rb             WB, IHC, IF, ELISA             20 uL
Vimentin 80232-1-RR             Rabbit monoclonal H, M, R             WB, IHC, IF, ELISA             20 uL
SNAI1 13099-1-AP             Rabbit polyclonal H, M, R             WB, IP, IHC, ELISA             20 uL
Mouse monoclonal             H, M, R, Pg             WB, IHC, ELISA             20 uL            
Fibronectin 66042-1-Ig
Mouse monoclonal             H, M, R             WB, IP, IHC, IF, ELISA             20 uL            
ZO-1 66452-1-Ig
Mouse monoclonal             H, Ca             WB, IHC, IF, ELISA             20 uL            
Twist 25465-1-AP
Rabbit polyclonal             H             WB, IP, IHC, IF, ELISA              20 uL            
MMP-9 10375-2-AP
Rabbit polyclonal             H, M, R             WB, IHC, IF, FC, ELISA             20 uL            
Claudin-1 13050-1-AP
Rabbit polyclonal             H, M, R, Ca             WB, IHC, IF, ELISA               20 uL            

Also see our 'EMT Essentials Antibody Kit' on the following page  https://www.ptglab.com/products/EMT-Essentials-Antibody-Kit-PK30014.htm


Store at -20°C. Stable for one year from the date of receipt. 

Background Information

The epithelial to mesenchymal transition (EMT) is a process by which epithelial cells transform into mesenchymal cells, allowing them to gain migratory and invasive properties. While a normal feature of embryonic development and tissue regeneration, EMT is often upregulated and becomes a key driver of metastases in several cancers including colorectal, breast, gastric, and melanoma. The EMT process itself typically involves the loss of epithelial markers such as E-cadherin and upregulation of mesenchymal makers such as fibronectin, N-cadherin, and vimentin. It also requires the expression of transcription factors such as snail, slug, and twist, which help to drive the transition into the mesenchymal phenotype. Matrix metalloproteinases (MMPs) additionally degrade the surrounding extracellular matrix and allow for the transformed cells to collectively migrate. The EMT process is commonly confirmed in cancer cells and tissue through western blot and immunohistochemistry. 

Standard Protocols

Click  here  to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30015, EMT Expanded Antibody Kit, Proteintech, IL, USA