Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HeLa cells, K-562 cells|
|Positive IHC detected in||human tonsillitis tissue, human pancreas cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, human tonsillitis tissue|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66321-1-Ig targets Fascin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Fascin fusion protein Ag6074|
|Full Name||fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC000521|
|Gene ID (NCBI)||6624|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
|Product Specific Protocols|
|WB protocol for Fascin antibody 66321-1-Ig||Download protocol|
|IHC protocol for Fascin antibody 66321-1-Ig||Download protocol|
|IF protocol for Fascin antibody 66321-1-Ig||Download protocol|
|FC protocol for Fascin antibody 66321-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.
The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.