Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B5G3

Cat no : 66321-1-Ig

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL



Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human pancreas cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, human tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66321-1-Ig targets Fascin in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fascin fusion protein Ag6074
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000521
Gene symbol FSCN1
Gene ID (NCBI) 6624
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It’s a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for Fascin antibody 66321-1-IgDownload protocol
IHC protocol for Fascin antibody 66321-1-IgDownload protocol
IF protocol for Fascin antibody 66321-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cytoskeleton (Hoboken)

The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.

Authors - Ivan Ramirez
humanWB,IF,IHC

Cell Prolif

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

Authors - Leiyu Hao
  • KD Validated