CoraLite®488-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1B5G3

Cat no : CL488-66321

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66321-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66321 targets Fascin in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fascin fusion protein Ag6074
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000521
Gene symbol FSCN1
Gene ID (NCBI) 6624
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
IF protocol for CL488 Fascin antibody CL488-66321Download protocol
FC protocol for CL488 Fascin antibody CL488-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols