CoraLite® Plus 488-conjugated Fascin Monoclonal antibody
Fascin Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B5G3
Cat no : CL488-66321
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human tonsillitis tissue |
Positive FC detected in | SH-SY5Y cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66321 targets Fascin in IF, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Fascin fusion protein Ag6074 |
Full Name | fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) |
Calculated molecular weight | 55 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC000521 |
Gene symbol | FSCN1 |
Gene ID (NCBI) | 6624 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Fascin antibody CL488-66321 | Download protocol |
FC protocol for CL Plus 488 Fascin antibody CL488-66321 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |