CoraLite®555-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B5G3

Cat no : CL555-66321

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:100-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66321 targets Fascin in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fascin fusion protein Ag6074
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000521
Gene Symbol FSCN1
Gene ID (NCBI) 6624
RRIDAB_2919683
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
IF protocol for CL555 Fascin antibody CL555-66321Download protocol
FC protocol for CL555 Fascin antibody CL555-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols