HSP27 Monoclonal antibody
HSP27 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3E7A1
Cat no : 66767-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HCT 116 cells, HSC-T6 cells, mouse liver tissue, U2OS cells, A549 cells, HeLa cells, HepG2 cells, HuH-7 cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
66767-1-Ig targets HSP27 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | HSP27 fusion protein Ag27859 |
Full Name | heat shock 27kDa protein 1 |
Calculated molecular weight | 19-23 kDa |
Observed molecular weight | 27 kDa |
GenBank accession number | BC012768 |
Gene symbol | HSPB1 |
Gene ID (NCBI) | 3315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HSP27 antibody 66767-1-Ig | Download protocol |
IHC protocol for HSP27 antibody 66767-1-Ig | Download protocol |
IF protocol for HSP27 antibody 66767-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Rep SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. |