Biotin-conjugated Human IgA Heavy Chain Polyclonal antibody

Human IgA Heavy Chain Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC

Conjugate

Biotin Fluorescent Dye

Cat no : Biotin-11449

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, IgA1, IGHA1

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Validation Data Gallery

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  IHC staining of human tonsillitis using Biotin-11449

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-11449 (Human IgA Heavy Chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

Biotin-11449 targets Human IgA Heavy Chain in IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Human IgA Heavy Chain fusion protein Ag2007
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated molecular weight: 496 aa, 53 kDa
• Observed molecular weight: 53 kDa
• GenBank accession number: BC016369
• Gene symbol: IGHA1
• Gene ID (NCBI): 3493
• Conjugate: Biotin Fluorescent Dye
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).