Human IgA Heavy Chain Polyclonal antibody

Human IgA Heavy Chain Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11449-1-AP

Synonyms

IGHA1



Tested Applications

Positive WB detected inhuman plasma tissue, human lung tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11449-1-AP targets Human IgA Heavy Chain in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Human IgA Heavy Chain fusion protein Ag2007
Full Name immunoglobulin heavy constant alpha 1
Calculated molecular weight 496 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC016369
Gene symbol IGHA1
Gene ID (NCBI) 3493
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Protocols

Product Specific Protocols
WB protocol for Human IgA Heavy Chain antibody 11449-1-APDownload protocol
IHC protocol for Human IgA Heavy Chain antibody 11449-1-APDownload protocol
IF protocol for Human IgA Heavy Chain antibody 11449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Anim Sci J

Effects of 7S and 11S on the intestine of weaned piglets after injection and oral administration of soybean antigen protein.

Authors - Chenglu Peng
mouseWB

Int Immunopharmacol

Altered microbial community structure in PI3Kγ knockout mice with colitis impeding relief of inflammation: Establishment of new indices for intestinal microbial disorder.

Authors - Yi Li
humanIHC

J Infect

Immune responses and residual SARS-CoV-2 in two critically ill COVID-19 patients before and after lung transplantation.

Authors - Yan Yan