JTV1 Monoclonal antibody

JTV1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66848-1-Ig

Synonyms

AIMP2, JTV1, JTV1 gene, P38, PRO0992, Protein JTV 1

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Validation Data Gallery

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  WB analysis using 66848-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66848-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 66848-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66848-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Product Information

66848-1-Ig targets JTV1 in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: JTV1 fusion protein Ag0675
• Full Name: JTV1 gene
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 32-36 kDa
• GenBank accession number: BC002853
• Gene symbol: JTV1
• Gene ID (NCBI): 7965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.