KI67 Antibody

KI67 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19972-1-AP

Synonyms



Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

19972-1-AP targets KI67 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
Observed molecular weight
GenBank accession number NM_002417
Gene symbol MKI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MKI67 gene encodes a nuclear protein, antigen KI-67, which was identified by monoclonal antibody Ki-67. Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (PMID: 8227122). The antigen Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors (PMID: 8227122).

Publications

SpeciesApplicationTitle
humanIHC

J Biol Chem

Identification of a small-molecule compound that inhibits homodimerization of oncogenic NAC1 protein and sensitizes cancer cells to anticancer agents.

Authors - XiaoHui Wang
humanWB,IHC

Cell Death Dis

PD-L1 promotes tumor growth and progression by activating WIP and β-catenin signaling pathways and predicts poor prognosis in lung cancer.

Authors - Wendan Yu
humanIHC

Exp Ther Med

Triptolide inhibits vascular endothelial growth factor-mediated angiogenesis in human breast cancer cells.

Authors - Huantao Liu
human

Oncol Lett

Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.

Authors - Qun Lu
humanIHC

Oncol Lett

Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models.

Authors - Ming Zhao
humanIHC

Med Oncol

Decreased Warburg effect induced by ATP citrate lyase suppression inhibits tumor growth in pancreatic cancer.

Authors - Haifeng Zong