MAP2 Monoclonal antibody, PBS Only

MAP2 Monoclonal Antibody for IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3E6

Cat no : 67015-1-PBS

Synonyms

MAP 2, MAP2, MAP2A, MAP2B, MAP2C

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Validation Data Gallery

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  FC experiment of Neuro-2a using 67015-1-Ig (same clone as 67015-1-PBS)

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 67015-1-Ig (same clone as 67015-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67015-1-PBS targets MAP2 in IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAP2 fusion protein Ag11349
• Full Name: microtubule-associated protein 2
• Calculated Molecular Weight: 200 kDa
• GenBank Accession Number: BC038857
• Gene Symbol: MAP2
• Gene ID (NCBI): 4133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.